What is TOOS (TOOS sodium salt)?
TOOS is short for sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropane sulfonate. It is also called TOOS sodium salt. It is a white, dry powder. It melts easily in water. It has a formula C₁₂H₁₈NNaO₄S. It weighs about 295.3 grams per mole. It is steady when kept dry and cool. TOOS works well in testing enzymes like peroxidase. It reacts fast and shows a sharp color change. That is why it is used often in labs.
How TOOS and MBTH work together?
TOOS forms a bright dye when used with MBTH. MBTH stands for 3-methyl-2-benzothiazolinone hydrazone. When MBTH is added to a sample with an enzyme, it changes. It forms a small piece called a free radical. That piece reacts with TOOS. The result is a colorless item at first. Then, it turns into a blue-violet color. This reaction helps labs measure things like blood sugar. The darker the color, the higher the result. TOOS is better than old materials like 4-AA.
TOOS (TOOS sodium salt) Reaction
TOOS sodium salt is often used in labs. It is safe and easy to mix in water. It reacts quickly in tests. It works with MBTH and enzymes to show clear color. This makes it useful in measuring blood sugar, uric acid, and more. When used in a sample, the enzyme acts first. That enzyme creates a chemical called hydrogen peroxide. MBTH reacts with this and forms a free radical. That radical and TOOS create a colorless link. Soon, this link changes into a blue-violet shade. That shade shows the final result. The stronger the color, the higher the value of the test. This method gives sharp, stable results. It also works in machines and manual tests.
TOOS is stable in powder form. Once it is mixed in water, it must be used fast. Always store it in a dry, dark place. The powder should be kept in sealed bottles. The storage range is 2°C to 8°C. In labs, users must wear gloves and use clean tools. If the powder touches skin or eyes, rinse well with water. It should never be used in food or medicine. It is made for lab use only. TOOS meets high purity standards. Most lab suppliers offer it with purity above 99%. It is trusted for its fast color change and stable reaction. That is why it is called a new Trinder reagent.
TOOS Sodium Salt Data
Property |
Value |
CAS Number |
82692-93-1 |
Formula |
C₁₂H₁₈NNaO₄S |
Molecular Weight |
295.3 g/mol |
Appearance |
White powder |
Water Solubility |
Over 100 mg/mL |
DMSO Solubility |
47 mg/mL or more |
Absorption Peak |
555–590 nm |
Reacts With |
MBTH and enzymes |
Product Type |
Trinder reagent |
Common Use |
Lab diagnostics |
Use Cases in TOOS (TOOS sodium salt) Diagnostics
TOOS is used in many blood tests. It helps in checking glucose levels. It works in cholesterol tests too. It is also used to test uric acid. Some labs use it for liver enzymes. These tests use enzymes like glucose oxidase and peroxidase. TOOS reacts with the enzyme to show a color change. This color is easy to read in machines. TOOS gives stronger color than older compounds. That makes it a better choice in health kits. Many labs around the world use TOOS in daily tests.
Safety and Storage
TOOS is safe if stored the right way. Keep it away from heat, air, and moisture. Store in a cool, dry place. Use sealed bottles. Do not keep it in sunlight. Store between 2°C to 8°C. Wear gloves when handling TOOS. If it touches skin, wash with water. If it gets in eyes, rinse well. TOOS is not for food, drug, or human use. It is only for lab work. Read the safety sheet before use. Keep it away from children. Use clean tools every time.
How to Use TOOS in Enzyme Tests
- First, take a clean glass or plastic tube.
- Add your enzyme sample to the tube.
- Add buffer solution as needed.
- Add MBTH in correct amount.
- Then add TOOS powder or solution.
- Mix the tube and keep it at room temperature.
- Wait for color to appear (blue-violet).
- Read the color using a spectrophotometer.
- Use the reading to check sugar, cholesterol, or enzyme levels.
- Always follow the test kit steps fully.
Advantages of Using TOOS
TOOS sodium salt gives many strong benefits in lab tests. It mixes fast in water without lumps. It gives a deep and stable color in minutes. This helps labs read results without delay. TOOS is easier and safer than older test chemicals. It is easy to keep in cool, dry places. It stays pure for a long time and works well in kits. You can use TOOS in both small labs and large machines. Many enzymes react well with TOOS, which makes it useful in different tests.
TOOS gives clear and clean output in blood tests. It helps check sugar, cholesterol, and enzyme levels. Labs prefer TOOS because it saves time and gives quick results. It works in manual methods and also in big test systems. The high purity of TOOS means fewer errors. This helps improve trust in test results. When used right, TOOS gives safe, stable, and clear data. For labs that want fast and trusted outcomes, TOOS is a smart pick.
TOOS (TOOS sodium salt) is a top choice for lab tests. It is easy to use, stable, and shows clear color. It helps labs get fast and correct results. From glucose to cholesterol, TOOS helps measure key health levels. It works better than many older compounds. It fits well in kits and large systems. If you need a strong, pure lab reagent, choose TOOS. Contact a trusted supplier today and get TOOS for your lab tests. Make every test count with TOOS.
Frequently Asked Questions (FAQs) for TOOS (TOOS sodium salt)
What is TOOS sodium salt?
TOOS sodium salt is a lab reagent used in blood tests.
How does TOOS work in tests?
It reacts with MBTH and enzymes to show a blue-violet color.
Where is TOOS used?
It is used in glucose, cholesterol, and uric acid blood tests.
What makes TOOS better than 4-AA?
TOOS gives a stronger color, so results are easier to read.
Is TOOS easy to mix in water?
Yes. It mixes fast and clear in water.
How should I store TOOS powder?
Store it in a sealed bottle, cool and dry, between 2°C to 8°C.
Can TOOS be used in food or drugs?
No. It is only for research and lab use.
Does TOOS work in machines?
Yes. It works well in both manual and automated testing systems.