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L-α-glycerophosphate oxidase (GPO) is an enzyme used in medical and research tests. It helps measure glycerol-related compounds and supports both diagnostic kits and biochemical studies in many labs worldwide.
L-α-glycerophosphate oxidase (GPO) is an enzyme used in medical and research tests. It helps measure glycerol-related compounds and supports both diagnostic kits and biochemical studies in many labs worldwide.
L-α-glycerophosphate oxidase (GPO) is an enzyme that breaks down L-α-glycerophosphate into hydrogen peroxide and dihydroxyacetone phosphate. This reaction is important in energy pathways. Scientists use GPO in diagnostic kits, food testing, and metabolic research. The enzyme is mainly sourced from certain bacterial strains where it supports cell metabolism.
GPO has value beyond natural systems. In labs, it acts as a marker enzyme in clinical tests. Its strong activity makes it helpful for glucose monitoring and lipid studies. Researchers rely on its stable properties to design reliable diagnostic products.
The enzyme is a flavoprotein that contains flavin adenine dinucleotide (FAD) as a cofactor. This cofactor is needed for its oxidase activity. The protein is usually a homodimer, which means it has two identical subunits. Each subunit binds to one FAD molecule.
The active site of GPO is where L-α-glycerophosphate binds and is oxidized. Structural studies show that this site has conserved amino acids that help in stabilizing the reaction. Knowing its structure is important for designing biosensors and diagnostic kits.
The main function of GPO is to oxidize L-α-glycerophosphate. During this reaction, the enzyme produces hydrogen peroxide. This product can be measured easily, which makes GPO useful in test kits. Hydrogen peroxide formation is linked with many detection methods.
In metabolism, the enzyme supports energy conversion by forming dihydroxyacetone phosphate. This compound then enters glycolysis and supports energy generation in cells. The dual role of helping metabolism and enabling testing makes GPO very important.
GPO is widely used in biochemical and medical research. Its activity is a marker for measuring glycerol pathways in microbes. In clinical studies, GPO helps detect metabolic disorders. It is also used to track lipid metabolism, which is important in diabetes research.
Research groups also study GPO as part of bacterial virulence factors. For example, in some pathogens, GPO generates hydrogen peroxide, which damages host tissues. This makes it an important target in infectious disease studies (NCBI).
GPO is used in diagnostic kits for measuring triglycerides and glycerol. The enzyme produces hydrogen peroxide, which reacts with color indicators. The color change gives a measurable result. This simple process is applied in clinical labs worldwide.
Testing with GPO is reliable for monitoring lipid levels in patients. High triglyceride or glycerol levels may show metabolic imbalance. Regular tests with GPO-based kits help track such conditions.
GPO is also used as a reference enzyme in many labs. Because its activity can be measured clearly, it helps standardize test reactions. Scientists studying metabolic pathways add GPO as a benchmark.
It is also useful in enzyme kinetics studies. Students and researchers measure how fast GPO reacts under different conditions. This data helps in designing better assays and biosensors.
GPO is found in bacteria such as Streptococcus pneumoniae. In these microbes, GPO plays a role in metabolism and sometimes in pathogenesis. The bacterial enzyme is studied for both clinical and biochemical purposes.
Some soil bacteria also produce GPO naturally. Scientists isolate it from these organisms and then purify it for use in labs.
Most GPO used in diagnostics is produced by recombinant methods. Scientists insert the gene coding for GPO into E. coli or other hosts. These hosts then express the enzyme in large amounts.
Recombinant production helps make pure, high-quality enzyme. It also reduces dependence on natural sources. This way, companies can make GPO in bulk for commercial kits.
The enzyme is central to many colorimetric assays. Diagnostic kits that measure triglycerides or glycerol use GPO for generating hydrogen peroxide. A chromogen reacts with this peroxide, producing a measurable color change.
GPO-based kits are widely available in hospitals and research labs. They offer quick results and are easy to use.
GPO is also applied in biosensor design. Biosensors use enzymes fixed on electrodes to detect compounds. When GPO oxidizes its substrate, it produces hydrogen peroxide. This is detected as an electrical signal.
Such biosensors are being studied for real-time monitoring of blood lipid levels. They offer promise for portable testing devices.
GPO must be stored at low temperatures to keep its activity. Most suppliers recommend freezing at -20°C or below. Buffers with stabilizers like glycerol are often used. These prevent enzyme loss during long-term storage.
The enzyme is stable when protected from light and repeated thawing. pH also affects its stability. Most GPO preparations are stable between pH 6 and 8. Researchers must handle it carefully to prevent activity loss.
GPO offers accurate measurement of glycerol and triglycerides. Its strong signal production makes research assays more reliable. Many scientists prefer it because of reproducibility. With recombinant production, it is available in consistent batches.
Pharma companies use GPO for quality control and drug testing. It helps in monitoring metabolic markers during trials. GPO-based tests are quick and cost-friendly. This reduces time in clinical pipelines.
Future research focuses on engineered versions of GPO. Mutant forms may provide higher stability or activity. Scientists are exploring GPO variants for use in portable sensors and point-of-care devices.
GPO may also be applied in food testing, agriculture, and environmental monitoring. Its ability to measure glycerol-related compounds makes it useful in quality control. Demand is expected to rise in industrial biotech fields.
| Application Area | Role of GPO | Example Use Case |
| Clinical Diagnostics | Measures glycerol and triglycerides | Lipid profile tests |
| Research Labs | Standard enzyme in kinetics | Enzyme activity studies |
| Biosensors | Produces measurable signals | Portable lipid testing devices |
| Pharma | Tracks metabolic markers | Drug trials for diabetes |
| Food Industry | Detects glycerol in samples | Quality testing in beverages |
L-α-glycerophosphate oxidase (GPO) is more than an enzyme. It supports clinical tests, biosensors, and research studies. With stable production and wide applications, it is valuable in both science and industry. For high-quality GPO and related raw materials, contact AARMEDICA today.
L-α-glycerophosphate oxidase (GPO) is mainly used in diagnostic kits to measure triglycerides and glycerol levels. It is also used in biosensors, research labs, and as a marker enzyme in studies. Its hydrogen peroxide output allows easy measurement in clinical testing.
GPO is sourced from bacteria such as Streptococcus pneumoniae. Today, most GPO is produced recombinantly in hosts like E. coli. This ensures pure, stable, and large-scale supply for labs and commercial use.
GPO oxidizes L-α-glycerophosphate, producing hydrogen peroxide and dihydroxyacetone phosphate. The hydrogen peroxide formed is measurable by color change or electrical signals. This reaction makes the enzyme useful in test kits and biosensors.
GPO enables simple, fast, and reliable lipid testing. Its hydrogen peroxide product reacts with color agents, giving quick visual results. Hospitals and labs use GPO-based kits for regular patient monitoring.
Yes, GPO can be stored at -20°C or lower. Stabilizers like glycerol are added to protect it. Enzyme activity remains stable if kept frozen and not exposed to repeated thawing.
Biosensors using GPO detect glycerol or triglycerides by measuring hydrogen peroxide. The enzyme is fixed on electrodes, and the product signal is converted to data. These devices are being studied for portable blood testing.
Future uses may include food testing, agriculture, and advanced portable diagnostics. Scientists are also working on engineered GPO versions with higher stability. This could expand its industrial reach.
AARMEDICA offers high-quality GPO suitable for clinical and research use. The company provides stable batches, reliable sourcing, and trusted service. Partnering with AARMEDICA helps labs and industries meet their testing needs with confidence.
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